mature human lymphocytes  (ATCC)

Journal: Biomaterials Research

Article Title: Rescue Radiosensitization of Pancreatic Cancer via PD-L1/TGF-β1 Dual-Blockade Nanotherapy as Evaluated in 3-Dimensional Microtumors

doi: 10.34133/bmr.0335

Figure Lengend Snippet: The biological multifunctional therapeutic effect of PFD@NGHP was assessed through a comprehensive evaluation. (A) Establishment schematic of Transwell assay system to evaluate the proliferation inhibition of PFD@NGHP. (B) Cell viability of PSCs determined by Transwell assay system. PFD@NGHP exhibited the most potent inhibitory profile. (C) Cell viability of different cell lines (PANC-1 cells, hPSCs, PANC02 cells, and mPSCs) was assessed via CCK-8 kit after different treatments including combination therapy or monotherapy. (D) Expression levels of collagen I, TGF-β1, α-SMA and α-tubulin in hPSC and mPSC lines treated under different conditions for 48 h, as determined by Western blotting. Con, control. (E) TGF-β1 secretion of hPSC and human cell coculture system (H-Co) under different conditions was evaluated by ELISA. Irradiated (RT+) cells and unirradiated (RT−) cells were both analyzed. (F) TGF-β1 secretion of mPSC and mouse cell coculture system (M-Co) under different conditions was evaluated by ELISA. Irradiated cells and unirradiated cells were both analyzed. (G) FCM analysis of PD-L1-targeting by NGHP in PANC-1 and PAN02 cells. NC, negative control. (H) ADCC assay was used to assess the functional status of αPD-L1 in NGHP. ADCC in PANC-1 and IR-PANC-1 (irradiated PANC-1) cells, mediated by different samples (“mNG” was mPEGS-nanogel), was induced using PBMC. All data are exhibited as the means ± SD ( n = 3), and the inserted asterisks indicate statistically significant differences based on * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The human peripheral blood mononuclear cells (PBMCs) were purchased from iXCells Biotechnologies (CA, USA).

Techniques: Transwell Assay, Inhibition, CCK-8 Assay, Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Irradiation, Negative Control, ADCC Assay, Functional Assay

Journal: Cell Communication and Signaling : CCS

Article Title: The metabolite α-ketoglutarate induces AIM2-dependent PANoptosis through demethylase TET2

doi: 10.1186/s12964-026-02740-3

Figure Lengend Snippet: Sepsis induced upregulation of IDH1-α-KG. a Schematic diagram of the TCA cycle; b Metabolomic screening of metabolite level changes in mouse PBMCs ( n = 4); c α-KG levels in PBMCs ( n = 4); d Citric acid levels in PBMCs ( n = 4); e Succinic acid levels in PBMCs ( n = 4); f Itaconic acid levels in PBMCs ( n = 4); g α-KG levels in PBMCs of sepsis ( n = 16) and healthy ( n = 11) patient; h Changes in PBMCs α-KG levels following LPS intraperitoneal injection ( n = 5); i. Changes in PBMCs α-KG levels in CLP-induced septic mice ( n = 5); j , k IDH1 expression in the GSE54514 dataset; l IDH1 protein expression in lung tissues of endotoxemia and CLP models ( n = 3); m , n IDH1 mRNA expression in lung tissues of endotoxemia and CLP models ( n = 6). o Immunohistochemical detection of IDH1 expression in mouse lung tissues

Article Snippet: Peripheral mononuclear macrophages (PBMCs) were separated from blood samples by using human peripheral blood mononuclear cell isolation medium (P9010, Solarbio) or mouse peripheral blood mononuclear cell isolation medium (P6340, Solarbio).

Techniques: Metabolomic, Injection, Expressing, Immunohistochemical staining